![]() Since there are significant differences in the quality of mAb clones, and particularly in their binding activity, an easy method for quick and low-cost comparison of various mAb clones was developed. Selecting the “right” monoclonal antibody(mAb) for an immunoaffinity-based application can be tricky, as many mAb producers offer a wide range of mAb clones against molecular structures of interest. Visit for more related articles at Journal of Analytical & Bioanalytical Techniques This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Studentska 573, 532 10 Pardubice, Czech Republic Tel: +420 46 6037700 E-mail: date: JAccepted date: JPublished date: June 28, 2013Ĭitation: Svobodova Z, Jankovicova B, Horak D, Bilkova Z (2013) Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies. 2, 162 06 Prague 6, Czech Republic *Corresponding Author: Svobodova Zuzanaĭepartment of Biological and Biochemical Sciencesįaculty of Chemical Technology, University of Pardubice Svobodova Z 1 *, Jankovicova B 1, Horak D 2 and Bilkova Z 1ġ Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech RepublicĢ Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies
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